The KLHL3 Polyclonal Antibody (CAB13771) is a valuable tool for researchers studying the KLHL3 protein, a member of the kelch-like protein family known to be involved in various cellular processes. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications.KLHL3 is a substrate-specific adaptor of the Cullin-3 E3 ubiquitin ligase complex, playing a crucial role in the ubiquitination and degradation of target proteins. Dysregulation of KLHL3 has been implicated in various diseases, including hypertension and kidney disorders.
By using this antibody to detect and analyze KLHL3 expression in different cell types, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new targets for therapeutic interventions.With its high specificity and reliability in detecting KLHL3, the CAB13771 antibody is a valuable asset for studies in molecular biology, biochemistry, and disease research. Its ability to facilitate the investigation of KLHL3 function makes it a valuable tool for advancing our understanding of cellular processes and disease mechanisms.
Product Name:
KLHL3 Rabbit Polyclonal Antibody
SKU:
CAB13771
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human KLHL3 (NP_059111.2).
This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
26249
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using KLHL3 Rabbit pAb (CAB13771) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
