The KLHL20 Polyclonal Antibody (PACO10134) is a valuable tool for researchers studying KLHL20, a protein involved in the regulation of cellular processes such as protein degradation and cell cycle progression. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.KLHL20 is a substrate-specific adapter protein that forms part of the Cullin3-based E3 ubiquitin ligase complex, playing a role in the polyubiquitination and degradation of target proteins. Its involvement in regulating protein turnover and cell cycle progression makes it a promising target for further investigation in the fields of cell biology and cancer research.
By utilizing the KLHL20 Polyclonal Antibody, researchers can effectively detect and analyze KLHL20 protein levels in various cell types, allowing for a better understanding of its function and potential implications in disease processes. This antibody is an essential tool for exploring the complex mechanisms underlying protein degradation and cell cycle regulation, with implications for therapeutic development in diseases such as cancer.
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response and anterograde Golgi to endosome transport. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis (PubMed:20389280). The BCR(KLHL20) E3 ubiquitin ligase complex also specifically mediates 'Lys-33'-linked ubiquitination (PubMed:24768539). Involved in anterograde Golgi to endosome transport by mediating 'Lys-33'-linked ubiquitination of CORO7, promoting interaction between CORO7 and EPS15, thereby facilitating actin polymerization and post-Golgi trafficking (PubMed:24768539). Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. The BCR(KLHL20) E3 ubiquitin ligase complex acts as a regulator of neurite outgrowth by mediating ubiquitination and degradation of PDZ-RhoGEF/ARHGEF11 (PubMed:21670212). In case of tumor, the BCR(KLHL20) E3 ubiquitin ligase complex is involved in tumor hypoxia: following hypoxia, the BCR(KLHL20)complex mediates ubiquitination and degradation of PML, potentiating HIF-1 signaling and cancer progression (PubMed:21840486).
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a member of the kelch family of proteins, which is characterized by a 44-56 amino acid repeat motif. The kelch motif appears in many different polypeptide contexts and contains multiple potential protein-protein contact sites. Members of this family are present both throughout the cell and extracellularly, with diverse activities. [provided by RefSeq, Jul 2008]
