The KLF4 Rabbit Monoclonal Antibody is a highly specific tool for researchers studying KLF4, a key transcription factor involved in various cellular processes such as differentiation, proliferation, and stem cell pluripotency. This antibody, generated from rabbits, exhibits high affinity and specificity for human samples and has been rigorously validated for use in Western blot and immunohistochemistry applications.KLF4, also known as Krüppel-like factor 4, plays a crucial role in regulating gene expression and cell fate determination, making it a valuable target for investigating developmental biology, cancer, and stem cell research.
By targeting the KLF4 protein, researchers can analyze its expression levels and subcellular localization in different cell types, providing insights into its functions and interactions in various physiological and pathological conditions.With its superior performance and reliability, the KLF4 Rabbit Monoclonal Antibody is an essential tool for scientists seeking to elucidate the molecular mechanisms underlying KLF4-mediated processes and potential therapeutic targets for diseases associated with dysregulation of this transcription factor.
Product Name:
KLF4 Rabbit Monoclonal Antibody
SKU:
CAB13673
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human KLF4 (O43474).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
EZF; GKLF; KLF4
Positive Sample:
A549,A-431,HeLa,Mouse lung
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
55kDa
Observed MW:
55kDa
This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
9314
Clone Number:
ARC0721
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using KLF4 Rabbit mAb (CAB13673) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
