The KLF4 Polyclonal Antibody (CAB6640) is a specialized tool for researchers studying the transcription factor KLF4, involved in regulating gene expression and cell fate determination. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.KLF4, a key player in cellular differentiation and pluripotency, has been linked to various biological processes such as embryonic development, stem cell maintenance, and tumorigenesis. By specifically binding to the KLF4 protein, this antibody enables accurate detection and analysis in different cell types, offering valuable insights for studies in stem cell biology, cancer research, and developmental biology.
The critical role of KLF4 in gene regulation and cell reprogramming underscores its importance as a target for investigating disease mechanisms and potential therapeutic interventions. By elucidating the functions and regulatory pathways associated with KLF4, researchers can advance our understanding of biological processes and pave the way for innovative strategies in regenerative medicine and cancer therapy.
Product Name:
KLF4 Rabbit Polyclonal Antibody
SKU:
CAB6640
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of KLF4 (NP_001300981.1).
This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
9314
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using KLF4 Rabbit pAb (CAB6640) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
