The KLF1 Polyclonal Antibody (CAB10581) is a valuable tool for researchers studying KLF1, a transcription factor that plays a crucial role in the regulation of gene expression, particularly in erythropoiesis. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.KLF1 is known for its involvement in the development and differentiation of red blood cells, making it a key player in hemoglobin synthesis and erythrocyte maturation. By targeting the KLF1 protein, researchers can detect and analyze its expression in various cell types, providing valuable insights into its function in normal and diseased states.
The KLF1 Polyclonal Antibody is an essential tool for studies in hematology, developmental biology, and genetic disorders related to erythropoiesis. By elucidating the role of KLF1 in these processes, researchers can advance our understanding of blood cell formation and potentially uncover new therapeutic targets for blood-related disorders.
Product Name:
KLF1 Rabbit Polyclonal Antibody
SKU:
CAB10581
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KLF1 (NP_006554.1).
Sequence:
ALGP FPDT QDDF LKWW RSE
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
EKLF; EKLF/KLF1; KLF1
Positive Sample:
Rat plasma
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
38kDa
Observed MW:
38kDa
This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype.
Purification Method:
Affinity purification
Gene ID:
10661
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of Rat plasma, using KLF1 antibody (CAB10581) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
