The Kininogen-1 Polyclonal Antibody (CAB1670) is a valuable tool for researchers studying kininogen-1, a precursor protein involved in the kallikrein-kinin system. This antibody, raised in rabbits, is highly specific for human kininogen-1 and has been validated for use in Western blot and ELISA applications.Kininogen-1 is known for its role in the regulation of blood pressure, inflammation, and coagulation, making it a key target for studies in cardiovascular and inflammatory diseases. By detecting and analyzing kininogen-1 expression in various cell types, this antibody facilitates research into the mechanisms underlying these physiological processes.
The Kininogen-1 Polyclonal Antibody is essential for scientists investigating the potential therapeutic implications of targeting kininogen-1 in conditions such as hypertension, thrombosis, and inflammatory disorders. Its high reactivity and specificity make it an ideal tool for advancing our understanding of the kallikrein-kinin system and its involvement in human health and disease.
Product Name:
Kininogen 1 (KNG1) Rabbit Polyclonal Antibody
SKU:
CAB1670
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 148-427 of human Kininogen 1 (KNG1) (NP_000884.1).
This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. Bradykinin also functions as an antimicrobial peptide with antibacterial and antifungal activity. In contrast to HMWK, LMWK is not involved in blood coagulation. Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reduces or depletes angiotensin converting enzyme 2 (ACE2), which results in an increase in levels of des-Arg(9)-bradykinin, a bioactive metabolite of bradykinin that is associated with lung injury and inflammation. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3827
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of Rat lung, using Kininogen1(KNG1) antibody (CAB1670) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.