The KIF14 Polyclonal Antibody (CAB10275) is a valuable tool for researchers studying the kinesin family member 14 (KIF14) protein. KIF14 is a motor protein that plays a key role in cell division and is implicated in various cellular processes, including mitotic progression and cytokinesis. This antibody, generated in rabbits, is highly specific for human samples and is validated for use in Western blot applications.By binding to the KIF14 protein, this antibody enables the detection and analysis of KIF14 in a wide range of cell types, making it a versatile tool for studies in cell biology and cancer research.
Understanding the function and regulation of KIF14 is essential for elucidating its role in cellular processes and its potential as a therapeutic target in cancer treatment.Overall, the KIF14 Polyclonal Antibody is a valuable resource for researchers seeking to investigate the function of KIF14 and its implications in cellular processes and diseases, providing valuable insights into potential therapeutic strategies.
Product Name:
KIF14 Rabbit Polyclonal Antibody
SKU:
CAB10275
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1399-1648 of human KIF14 (NP_055690.1).
This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
9928
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using KIF14 Rabbit pAb (CAB10275) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
