The Kidins220 Polyclonal Antibody (PAC019898) is a valuable tool for research involving the Kidins220 protein, known for its role in neuronal signaling and neuroprotection. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the Kidins220 protein, researchers can accurately analyze and detect its presence in various cell types, making it ideal for studies in neuroscience and neurodegenerative diseases.Kidins220, also known as ankyrin repeat-rich membrane spanning (ARMS), is a key player in promoting neuronal survival and growth through its interactions with growth factor receptors and intracellular signaling pathways.
Its importance in neuroprotection and neuronal function highlights its potential as a target for therapeutic interventions in conditions such as Alzheimer's disease, Parkinson's disease, and stroke. Further exploration of Kidins220 activity is essential for advancing our understanding of neurobiology and developing novel treatment strategies for neurological disorders.
Antibody Name:
KIDINS220 Antibody (PACO19898)
Antibody SKU:
PACO19898
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:1000-1:2000, WB:1:200-1:1000
Species Reactivity:
Human, Rat
Immunogen:
Synthetic peptide of human KIDINS220
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Raji cells, Hela cells, Primary antibody: PACO19898(KIDINS220 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds.
Background:
Ankyrin repeat-rich membrane-spanning protein (ARMS), also designated kinase D-interacting substance 220 or Kidins220, is a highly conserved protein containing multiple domains, including four putative transmembrane domains and several ankyrin repeats. ARMS is expressed in regions rich in neurotrophin (Trk) and ephrin (Eph) receptors, such as the brain and neuroendocrine cells (where it concentrates at the tip of neurites) and in plastic areas of the adult brain. It is also detected in peripheral blood immature dendritic cells and PC12 cells. ARMS functions as a substrate for protein kinase D and is a downstream target for both Trk and Eph receptors. It is a highly conserved protein, which suggests it has an evolutionary conserved role. The gene encoding for the protein maps to chromosome 2p24.
Synonyms:
kinase D-interacting substrate, 220kDa
UniProt Protein Function:
KIDINS220: Promotes a prolonged MAP-kinase signaling by neurotrophins through activation of a Rap1-dependent mechanism. Provides a docking site for the CRKL-C3G complex, resulting in Rap1-dependent sustained ERK activation. May play an important role in regulating postsynaptic signal transduction through the syntrophin-mediated localization of receptor tyrosine kinases such as EPHA4. In cooperation with SNTA1 can enhance EPHA4-induced JAK/STAT activation. May play a role in neurotrophin- and ephrin- mediated neuronal outgrowth and in axon guidance during neural development and in neuronal regeneration. Modulates stress-induced apoptosis of melanoma cells via regulation of the MEK/ERK signaling pathway. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Membrane protein, multi-pass; Membrane protein, integral; Protein kinase, regulatory subunit
Chromosomal Location of Human Ortholog: 2p24
Cellular Component: nucleoplasm; protein complex; membrane; late endosome; integral to membrane; cytosol
Molecular Function:PDZ domain binding
Biological Process: activation of MAPKK activity; nerve growth factor receptor signaling pathway; in utero embryonic development; dendrite morphogenesis; cytoplasmic transport
