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alpha-Ketoglutarate Dehydrogenase (alpha-KGDH)Activity Assay Kit (MAES0236)

SKU:
MAES0236
Product Type:
Assay
Instrument:
Microplate Reader
Detection Method:
Colorimetric
Sample Type:
Serum, Plasma, Animal And Plant Tissue
Sensitivity:
8.3 U/L
Range:
8.3-42.3 U/L
Assay Time:
40 min
€479

Description

Product Name:α-Ketoglutarate Dehydrogenase(α-KGDH)Activity Assay Kit
Product Code:MAES0236
Sample Type:Serum, Plasma, Animal And Plant Tissue
Assay Time:40 min
Assay Type:Enzyme Activity
Synonyms:α-KGDH
Detection Instrument:Microplate reader (440-460 nm, optimum wavelength: 450 nm)
Detection Method:Colorimetric method
Sensitivity:8.3 U/L
Detection Range:8.3-42.3 U/L
Inter-assay CV:0.085
Intra-assay CV:0.015

α-ketoglutarate dehydrogenase (α-KGDH) is a key enzyme in the tricarboxylic acid (TCA) cycle. While the substrate being catalyzed by α-KGDH, NAD+ is reduced to NADH, and electrons are transfered to WST-8 under the action of hydrogen transmitter to produce yellow product. The activity of α-KGDH can be calculated by measuring the change of absorbance value at 450 nm

Storage:-20°C
Valid Period:12 months
Other Instruments Required:Incubator, Centrifuge

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