The Keap1 Polyclonal Antibody (CAB21724) is a critical tool for researchers studying the Keap1 protein, which plays a crucial role in oxidative stress response and the regulation of cellular redox balance. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.Keap1, also known as Kelch-like ECH-associated protein 1, functions as a sensor for oxidative stress and is involved in the activation of the transcription factor Nrf2, which regulates the expression of antioxidant genes. Dysregulation of the Keap1/Nrf2 pathway has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
By using the Keap1 Polyclonal Antibody, researchers can accurately detect and analyze Keap1 protein levels in cell samples, allowing for a better understanding of its role in oxidative stress and disease development. This antibody is an essential tool for studies in molecular biology, biochemistry, and therapeutic development aimed at targeting the Keap1/Nrf2 pathway for potential disease interventions.
Product Name:
KEAP1 Polyclonal Antibody
SKU:
CAB21724
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 320-510 of human KEAP1 (NP_036421.2).
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
9817
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type(WT) and KEAP1 Rabbit pAb knockout (KO) 293T cells, using [KO Validated] KEAP1 Rabbit pAb (CAB21724) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
