The KDM5B Polyclonal Antibody (CAB7772) is a valuable tool for researchers studying KDM5B, a key epigenetic regulator involved in gene expression and chromatin remodeling. This antibody, generated in rabbits, shows high specificity and sensitivity in detecting KDM5B in human samples, and has been validated for use in Western blot applications.KDM5B, also known as JARID1B, is a member of the Jumonji family of histone demethylases and plays a critical role in regulating gene expression by removing methyl groups from histone proteins. Dysregulation of KDM5B has been linked to various diseases, including cancer, neurodevelopmental disorders, and metabolic syndromes, making it a promising target for therapeutic interventions.
By utilizing the KDM5B Polyclonal Antibody, researchers can accurately assess KDM5B protein levels and activity in different cell types and tissues, providing valuable insights into its function and potential implications in disease mechanisms. This antibody is a valuable asset for studies in epigenetics, cancer biology, and drug discovery, offering new opportunities for advancing our understanding of gene regulation and identifying novel therapeutic targets.
Product Name:
[KO Validated] KDM5B Rabbit Polyclonal Antibody
SKU:
CAB7772
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 784-883 of human KDM5B (NP_006609.3).
This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
10765
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts from normal (control) and KDM5B knockout (KO) 293T cells, using KDM5B antibody (CAB7772) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
