The KDM4B/JMJD2B Rabbit Monoclonal Antibody (CAB6670) is a essential tool for researchers studying the functions of lysine-specific demethylase 4B (KDM4B) and jumonji domain-containing protein 2B (JMJD2B) in cellular processes such as gene expression and chromatin remodeling. This highly specific antibody, generated in rabbits, is suitable for use in various applications including Western blot, immunohistochemistry, and immunofluorescence.KDM4B and JMJD2B are members of the Jumonji C domain-containing histone demethylase family, involved in the removal of methyl groups from lysine residues on histone proteins. These proteins play a crucial role in epigenetic regulation, influencing gene expression patterns and cellular differentiation.
By targeting KDM4B and JMJD2B, researchers can gain insights into the mechanisms underlying these processes and their relevance in diseases such as cancer and neurological disorders.The CAB6670 antibody offers high specificity and sensitivity for detecting KDM4B and JMJD2B proteins in various cell types and tissue samples, enabling detailed analysis of their expression levels and subcellular localization. Its reliable performance makes it an invaluable tool for studies exploring the roles of KDM4B and JMJD2B in epigenetic regulation and their potential as therapeutic targets in disease intervention.
Product Name:
KDM4B/JMJD2B Rabbit Monoclonal Antibody
SKU:
CAB6670
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human KDM4B/JMJD2B (O94953).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 10μg-15μg of Chromatin
Synonyms:
MRD65; JMJD2B; TDRD14B; KDM4B/JMJD2B
Positive Sample:
HCT 116,Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
122kDa
Observed MW:
150kDa
Enables histone H3-methyl-lysine-36 demethylase activity and histone H3-methyl-lysine-9 demethylase activity. Involved in histone H3-K36 demethylation and histone H3-K9 demethylation. Located in cytosol and nucleoplasm. Implicated in autosomal dominant non-syndromic intellectual disability; breast cancer; colorectal cancer; malignant peripheral nerve sheath tumor; and stomach cancer. Biomarker of several diseases, including alopecia areata; lung cancer; medulloblastoma; prostate cancer; and stomach cancer.
Purification Method:
Affinity purification
Gene ID:
23030
Clone Number:
ARC1416
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using KDM4B/JMJD2B Rabbit mAb (CAB6670) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
