The SMAD4 Polyclonal Antibody is a valuable tool for researchers studying the SMAD4 protein, a key regulator in the TGF-beta signaling pathway. This antibody is raised in rabbits and has been validated for use in Western blot applications, providing reliable detection and analysis of SMAD4 in a variety of cell types. SMAD4 is a critical transcription factor involved in cell growth, differentiation, and apoptosis, making it a crucial target for studies in cancer research and developmental biology. Aberrant SMAD4 signaling has been implicated in various diseases, including cancer and developmental disorders, underscoring the importance of understanding its function.
By using the SMAD4 Polyclonal Antibody, researchers can gain insights into the role of SMAD4 in cellular processes and disease pathogenesis, paving the way for the development of targeted therapies and interventions. This antibody is highly specific for human samples, making it an ideal choice for investigations into the molecular mechanisms of TGF-beta signaling and its implications for human health.
Product Name:
[KD Validated] Smad4 Polyclonal Antibody
SKU:
CAB21487
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 160-450 of human Smad4 (NP_005350.1).
This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasing blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
Purification Method:
Affinity purification
Gene ID:
4089
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HCT116 cells, using [KD Validated] Smad4 Rabbit pAb (CAB21487) at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
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