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[KD Validated] Raf1 Polyclonal Antibody (CAB23679)

SKU:
CAB23679
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €529
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KD Validated] Raf1 Polyclonal Antibody
SKU:CAB23679
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant protein of human Raf1.
Sequence:IRQL LLFP NSTI GDSG VPAL PSLT MRRM RESV SRMP VSSQ HRYS TPHA FTFN TSSP SSEG SLSQ RQRS TSTP NVHM VSTT LPVD SRMI EDAI RSHS ESAS PSAL SSSP NNLS PTGW SQPK TPVP AQRE RAPV SGTQ EKNK IRPR GQRD SSYY WEIE ASEV M
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:5000
Synonyms:NS5; CRAF; Raf-1; c-Raf; CMD1NN; [KD Validated] Raf1
Positive Sample:293T,Mouse lung
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Mitochondrion, Nucleus.
Calculated MW:73kDa
Observed MW:75kDa

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Purification Method:Affinity purification
Gene ID:5894
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Mouse lung, using [KD Validated] Raf1 Rabbit pAb (CAB23679) at 1:4000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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