The NPHS1 Polyclonal Antibody (AB10001) is a valuable tool for researchers studying NPHS1, a protein crucial for maintaining the structure and function of the glomerular filtration barrier in the kidney. This antibody, generated in rabbits, specifically targets human NPHS1 and has been validated for use in various applications, including Western blot and immunohistochemistry. NPHS1, also known as nephrin, is a key player in the regulation of kidney function and the development of nephrotic syndrome, a serious kidney disorder characterized by proteinuria and edema. By targeting NPHS1 with this antibody, researchers can gain insights into the role of this protein in kidney health and disease, paving the way for potential diagnostic and therapeutic advancements in the field of nephrology.
Whether investigating the molecular mechanisms underlying kidney dysfunction or developing novel treatments for kidney diseases, the NPHS1 Polyclonal Antibody is a valuable resource for researchers seeking to unravel the complexities of renal biology. Its high specificity and sensitivity make it an essential tool for studies focused on understanding and addressing kidney-related disorders.
Product Name:
[KD Validated] NPHS1 Polyclonal Antibody
SKU:
CAB21985
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1019-1241 of human NPHS1 (NP_004637.1).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
135kDa
Observed MW:
200kDa
This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.
Purification Method:
Affinity purification
Gene ID:
4868
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts from wild type(WT) and NPHS1 knockdown (KD) A-549 cells, using NPHS1 antibody (CAB21985) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
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