The MAD1/MAD1L1 Polyclonal Antibody (AB10019) is a valuable tool for research involving the MAD1 and MAD1L1 proteins, which are essential components of the spindle assembly checkpoint (SAC) pathway. This pathway plays a critical role in ensuring accurate chromosome segregation during cell division, making it a key target for studying cell cycle regulation and genomic stability.Raised in rabbits, this antibody is highly specific and reactive with human samples, making it ideal for use in Western blot applications to detect and analyze the expression of MAD1 and MAD1L1 proteins in various cell types.
Its validation for use in research allows for reliable and accurate results, making it a valuable asset for studies in cell biology, cancer research, and drug development.The MAD1/MAD1L1 Polyclonal Antibody provides researchers with a powerful tool to investigate the function and regulation of the SAC pathway, offering insights into the mechanisms of chromosome segregation and potential targets for cancer therapy. Its ability to specifically bind to MAD1 and MAD1L1 proteins makes it a versatile tool for understanding the complex processes of cell division and genomic stability.
Product Name:
[KD Validated] MAD1/MAD1L1 Polyclonal Antibody
SKU:
CAB22000
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 351-718 of human MAD1/MAD1L1 (NP_003541.2).
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
8379
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using [KD Validated] MAD1/MAD1L1 Rabbit pAb (CAB22000) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
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