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[KD Validated] IL13 Polyclonal Antibody (CAB22112)

SKU:
CAB22112
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
$167 - $635
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KD Validated] IL13 Polyclonal Antibody
SKU:CAB22112
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-146 of human IL13 (NP_002179.2).
Sequence:LTCL GGFA SPGP VPPS TALR ELIE ELVN ITQN QKAP LCNG SMVW SINL TAGM YCAA LESL INVS GCSA IEKT QRML SGFC PHKV SAGQ FSSL HVRD TKIE VAQF VKDL LLHL KKLF REGQ FN
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:P600; IL-13; [KD Validated] IL13
Positive Sample:MCF7
Conjugate:Unconjugated
Cellular Localization:cytoplasm, external side of plasma membrane, extracellular region, extracellular space
Calculated MW:16kDa
Observed MW:13-16kDa

This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.

Purification Method:Affinity purification
Gene ID:3596
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type(WT) and IL13 knockdown (KD) MCF7 cells, using [KD Validated] IL13 Rabbit pAb (CAB22112) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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