The GPX4 Polyclonal Antibody is a versatile tool for researchers studying GPX4, a key enzyme involved in lipid peroxidation and ferroptosis, a form of regulated cell death. The antibody, raised in rabbits, has been validated for use in various applications, including Western blot and immunohistochemistry, and is highly reactive with human samples. By targeting the GPX4 protein, this antibody enables precise detection and analysis in different cellular contexts, making it an essential tool for studying oxidative stress, cell death mechanisms, and potential therapeutic interventions in cancer and neurodegenerative diseases.
GPX4 is a critical regulator of redox homeostasis and cell survival, playing a vital role in protecting cells from oxidative damage and ferroptotic cell death. Dysregulation of GPX4 has been implicated in various diseases, including cancer, neurodegeneration, and ischemia-reperfusion injury. By understanding the mechanisms by which GPX4 functions, researchers can identify novel treatment strategies targeting this enzyme and its associated pathways, ultimately leading to the development of more effective therapies for oxidative stress-related disorders.
Product Name:
[KD Validated] GPX4 Polyclonal Antibody
SKU:
CAB21440
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-197 of human GPX4 (NP_002076.2).
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Transcript variants resulting from alternative splicing or use of alternate promoters have been described to encode isoforms with different subcellular localization.
Purification Method:
Affinity purification
Gene ID:
2879
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KD Validated] GPX4 Rabbit pAb (CAB21440) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
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