The EIF4E Polyclonal Antibody is a powerful research tool designed for studies involving EIF4E, a key protein involved in the regulation of mRNA translation and cell growth. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for Western blot applications.EIF4E is a critical component of the eukaryotic translation initiation complex and plays a vital role in controlling the rate of protein synthesis. Dysregulation of EIF4E has been implicated in various diseases, including cancer, where increased levels of EIF4E are associated with tumor progression and metastasis.
By targeting EIF4E with this polyclonal antibody, researchers can accurately detect and analyze the expression of EIF4E in a variety of cell types, shedding light on its functions and potential implications in disease pathology. This antibody is a valuable tool for investigations in molecular biology, cancer research, and drug development, providing valuable insights into the role of EIF4E in cellular processes.
Product Name:
[KD Validated] eIF4E Polyclonal Antibody
SKU:
CAB22681
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human eIF4E. (NP_001959.1).
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1977
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KD Validated] eIF4E Rabbit pAb (CAB22681) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
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