[KD Validated] COX IV Polyclonal Antibody (CAB22871)
The KD-Validated Cox-IV Polyclonal Antibody offered by AssayGenie is a high-quality tool for researchers studying the expression and function of the Cox-IV protein. Cox-IV is a key subunit of cytochrome c oxidase, the final enzyme in the respiratory electron transport chain. This antibody, produced in rabbits, has been rigorously validated for specificity and sensitivity in Western blotting applications, making it a reliable choice for experiments involving Cox-IV detection.With its high reactivity with human samples, the KD-Validated Cox-IV Polyclonal Antibody is ideal for investigating the role of Cox-IV in cellular respiration and energy production.
By targeting Cox-IV, researchers can gain insights into mitochondrial function and dysfunction, which are implicated in various diseases such as metabolic disorders, neurodegenerative conditions, and cancer.Overall, this antibody is a valuable tool for studies focusing on mitochondrial biology, energy metabolism, and oxidative phosphorylation pathways. Its specificity and reliability make it a trusted reagent for researchers seeking to explore the intricate mechanisms of Cox-IV and its implications in health and disease.
Product Name:
[KD Validated] COX IV Polyclonal Antibody
SKU:
CAB22871
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 23-98 of human COX IV. (NP_001852.1).
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
1327
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KD Validated] COX IV Rabbit pAb (CAB22871) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
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