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[KD Validated] COMT Polyclonal Antibody (CAB23600)

SKU:
CAB23600
$167
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Description

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Product Name:[KD Validated] COMT Polyclonal Antibody
SKU:CAB23600
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 51-271 of human COMT. (NP_000745.1).
Sequence:MGDT KEQR ILNH VLQH AEPG NAQS VLEA IDTY CEQK EWAM NVGD KKGK IVDA VIQE HQPS VLLE LGAY CGYS AVRM ARLL SPGA RLIT IEIN PDCA AITQ RMVD FAGV KDKV TLVV GASQ DIIP QLKK KYDV DTLD MVFL DHWK DRYL PDTL LLEE CGLL RKGT VLLA DNVI CPGA PDFL AHVR GSSC FECT HYQS FLEY REVV DGLE KAIY KGPG SEAG P
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:HEL-S-98n; [KD Validated] COMT
Positive Sample:THP-1,293T
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Extracellular side, Single-pass type II membrane protein.
Calculated MW:30kDa
Observed MW:28,30kDa

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.

Purification Method:Affinity purification
Gene ID:1312
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from THP-1 cells using [KD Validated] COMT Rabbit pAb (CAB23600) at 1:700 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)( AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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