The KD Validated BUB1 Monoclonal Antibody is a valuable tool for researchers studying the BUB1 protein, a key player in the regulation of cell division. This antibody, generated using hybridoma technology, exhibits high specificity and sensitivity in detecting BUB1 in various experimental settings.BUB1 is a crucial component of the mitotic spindle assembly checkpoint, ensuring accurate chromosome segregation during cell division. Dysregulation of BUB1 has been linked to chromosomal instability and tumorigenesis, making it a promising target for cancer therapy development.With its KD validation, this antibody guarantees reliable and reproducible results in assays such as immunofluorescence, immunoprecipitation, and flow cytometry.
Its versatility and accuracy make it an indispensable tool for researchers investigating cell cycle regulation, cancer biology, and potential therapeutic strategies targeting BUB1. Overall, the KD Validated BUB1 Monoclonal Antibody offers researchers a reliable and effective means to explore the functions and mechanisms of the BUB1 protein, advancing our understanding of cell division and its implications in disease.
Product Name:
[KD Validated] BUB1 Monoclonal Antibody
SKU:
CAB23198
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant protein of human BUB1.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
BUB1A; BUB1L; hBUB1; MCPH30; [KD Validated] BUB1
Positive Sample:
HEL,293T
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Nucleus, centromere, kinetochore.
Calculated MW:
115KDa/120KDa/122KDa
Observed MW:
122kDa
This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
699
Clone Number:
ARC59688
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HEL cells, using [KD Validated] BUB1 Rabbit mAb (CAB23198) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
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