The KCNMB3 Antibody (PAC019889) is a high-quality polyclonal antibody designed for research involving KCNMB3, a gene encoding a protein known as the big conductance calcium-activated potassium channel (BK channel) accessory subunit beta-3. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in various applications, including Western blot analysis.The KCNMB3 Antibody specifically targets the KCNMB3 protein, allowing for accurate detection and analysis in a variety of cell types. This makes it an ideal tool for researchers studying the role of KCNMB3 in ion channel function, calcium signaling, and other physiological processes. Additionally, the antibody's reliability and specificity make it well-suited for investigations in the fields of neurobiology, cardiovascular research, and beyond.
The BK channel, regulated by the KCNMB3 subunit, plays a crucial role in controlling cell excitability and neurotransmitter release, making it a key player in neuronal signaling and synaptic transmission. Dysregulation of BK channels has been implicated in various diseases, including epilepsy, hypertension, and neurological disorders. By targeting KCNMB3 with this antibody, researchers can gain valuable insights into the function and regulation of BK channels, potentially leading to the development of novel therapeutic strategies for treating associated conditions.
Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: Hepg2 cells, Primary antibody: PACO19889(KCNMB3 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute.
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO19889(KCNMB3 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming α subunit and the modulatory β subunit. The protein encoded by this gene is an auxiliary β subunit which may partially inactivate or slightly decrease the activation time of MaxiK α subunit currents. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 22.
Synonyms:
potassium large conductance calcium-activated channel, subfamily M β member 3
UniProt Protein Function:
KCNMB3: Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Alters the functional properties of the current expressed by the KCNMA1 channel. Isoform 2, isoform 3 and isoform 4 partially inactivate the current of KCNBMA. Isoform 4 induces a fast and incomplete inactivation of KCNMA1 channel that is detectable only at large depolarizations. In contrast, isoform 1 does not induce detectable inactivation of KCNMA1. Two or more subunits of KCNMB3 are required to block the KCNMA1 tetramer. Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB3 subfamily. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, multi-pass; Membrane protein, integralChromosomal Location of Human Ortholog: 3q26.3-q27Cellular Component: voltage-gated potassium channel complex; integral to plasma membrane; plasma membraneMolecular Function: calcium-activated potassium channel activity; potassium channel regulator activityBiological Process: regulation of action potential; synaptic transmission; generation of action potential; detection of calcium ion; blood coagulation; potassium ion transport
UniProt Protein Details:
NCBI Summary:
MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. The protein encoded by this gene is an auxiliary beta subunit which may partially inactivate or slightly decrease the activation time of MaxiK alpha subunit currents. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 22. [provided by RefSeq, Jul 2009]
