The KARS Polyclonal Antibody (CAB13772) is a valuable tool for researchers studying the enzyme Lysyl-tRNA synthetase (KARS) and its role in protein synthesis and cellular function. This antibody, generated in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to the KARS protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for investigations in molecular biology and cell signaling pathways.KARS is an essential enzyme responsible for attaching the amino acid lysine to its cognate tRNA molecule during protein synthesis.
Dysregulation of KARS expression or activity has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions. Research on KARS can provide valuable insights into the molecular mechanisms underlying these diseases and potential therapeutic targets for intervention. The KARS Polyclonal Antibody is a reliable tool for elucidating the functions and regulatory mechanisms of KARS in cellular processes, paving the way for advancements in biomedical research and drug development.
Product Name:
LysRS/KARS Rabbit Polyclonal Antibody
SKU:
CAB13772
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 298-597 of human LysRS/LysRS/KARS (NP_005539.1).
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3735
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using LysRS/KARS Rabbit pAb (CAB13772) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
