The KALRN Polyclonal Antibody (PAC06331) is a key tool for researchers studying KALRN, a protein involved in cell signaling and cytoskeleton organization. This antibody, generated in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By binding to the KALRN protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential tool for investigations in cell biology and cancer research.KALRN, also known as Kalirin, is a Rho guanine nucleotide exchange factor that plays a critical role in cytoskeletal dynamics and neuronal development. Dysregulation of KALRN has been implicated in various diseases, including cancer and neurological disorders, making it a promising target for therapeutic intervention.
Understanding the functions and interactions of KALRN is essential for unraveling its role in disease pathogenesis and identifying potential drug targets for treatment strategies.In conclusion, the KALRN Polyclonal Antibody (PAC06331) provides researchers with a reliable tool for studying the intricate functions of the KALRN protein in various biological processes. Its high reactivity and specificity, combined with its versatility in different research applications, make it an invaluable asset for advancing our understanding of KALRN-related diseases and developing novel therapeutic approaches.
IHC image of PACO63331 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO63331 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Promotes the exchange of GDP by GTP. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity.
Synonyms:
Kalirin (EC 2.7.11.1) (Huntingtin-associated protein-interacting protein) (Protein Duo) (Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain), KALRN, DUET DUO HAPIP TRAD
UniProt Protein Function:
Promotes the exchange of GDP by GTP. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity.
UniProt Protein Details:
NCBI Summary:
Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with the huntingtin-associated protein 1, which is a huntingtin binding protein that may function in vesicle trafficking. [provided by RefSeq, Apr 2016]
