K6-linkage Specific Polyubiquitin Rabbit Polyclonal Antibody (CAB18106)
The K6 Linkage-Specific Polyubiquitin rabbit polyclonal antibody (CAB18106) is a valuable tool for researchers studying the role of K6 linkage-specific polyubiquitin chains in various biological processes. This antibody, generated in rabbits, is highly specific for K6-linked polyubiquitin chains in human samples, making it an ideal choice for Western blot applications.K6 linkage-specific polyubiquitin chains play a critical role in protein degradation, signal transduction, and DNA repair processes. By targeting these specific chains, researchers can gain valuable insights into the mechanisms underlying these cellular processes.
The CAB18106 antibody allows for the detection and analysis of K6-linked polyubiquitin chains in various cell types, facilitating studies in immunology, cancer research, and other fields.Understanding the function of K6 linkage-specific polyubiquitin chains is essential for unraveling their role in disease development and progression. This antibody provides researchers with a reliable tool to further explore the implications of K6-linked polyubiquitin chains in various biological contexts, ultimately contributing to the advancement of scientific knowledge in the field.
Product Name:
K6-linkage Specific Polyubiquitin Rabbit Polyclonal Antibody
SKU:
CAB18106
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence containing polyubiquitinated protein (K6 linkage).
Tested Applications:
WBELISADB
Recommended Dilution:
WB,1:500 - 1:2000 DB,1:500 - 1:1000
Positive Sample:
RAW264.7,C6
Polyubiquitin chains regulate diverse cellular processes through the ability of ubiquitin to form chains of eight different linkage types. Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair. it has been reported that modification at Lys-6 inhibits ubiquitin-dependent protein degradation, a failure of the ubiquitin-proteasome system could play a role in initiating the formation of degradation-resistant PHF tangles.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using K6-linkage Specific Polyubiquitin Rabbit pAb (CAB18106) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
