K48-linkage Specific Ubiquitin Rabbit Monoclonal Antibody (CAB3606)
The K48 Linkage Specific Ubiquitin Rabbit Monoclonal Antibody (CAB3606) is a powerful tool for researchers studying the ubiquitination process, specifically targeting proteins tagged with K48-linked ubiquitin chains. This antibody, produced in rabbits, is highly specific and sensitive for detecting K48-linked ubiquitin in human samples. Ubiquitination is a crucial post-translational modification involved in regulating protein degradation, cell signaling, and various cellular processes. K48-linked ubiquitin chains, in particular, target proteins for proteasomal degradation. The CAB3606 antibody recognizes these specific ubiquitin chains, allowing researchers to study their role in various biological pathways.This antibody is validated for use in Western blot applications, enabling researchers to detect and quantify K48-linked ubiquitin conjugates in different cell types and experimental conditions.
Its high reactivity and specificity make it an essential tool for studies in cell biology, signal transduction, and disease mechanisms related to protein degradation.Overall, the K48 Linkage Specific Ubiquitin Rabbit Monoclonal Antibody (CAB3606) is a valuable resource for researchers interested in understanding the complex regulatory mechanisms of protein turnover through the ubiquitin-proteasome system. Its reliable performance and specificity make it an indispensable tool for investigations in molecular biology, biochemistry, and drug discovery.
Product Name:
K48-linkage Specific Ubiquitin Rabbit Monoclonal Antibody
SKU:
CAB3606
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Ubiquitin (P0CG47).
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
7314
Clone Number:
ARC0811
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using K48-linkage Specific Ubiquitin Rabbit mAb (CAB3606) at 1:1000 dilution.HeLa cells were treated by MG132(50 μM) at 37℃ for 90 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
