K29-linkage Specific Polyubiquitin Rabbit Polyclonal Antibody (CAB18198)
The K29 Linkage-Specific Polyubiquitin Rabbit Polyclonal Antibody (CAB18198) is a valuable tool for researchers studying protein ubiquitination pathways. This antibody is raised in rabbits and specifically detects proteins modified with K29-linked polyubiquitin chains. It is highly reactive with human samples and has been validated for use in various applications, including Western blotting.Ubiquitin modification plays a crucial role in regulating protein turnover, signal transduction, and DNA repair processes within cells. The K29 linkage is a less common form of ubiquitination compared to the more well-known K48 and K63 linkages, making this antibody particularly useful for studying unique aspects of the ubiquitin system.
By targeting proteins modified with K29-linked polyubiquitin chains, this antibody allows for the detection and analysis of specific protein modifications in various cell types and tissues. Researchers in the fields of cell biology, molecular biology, and cancer research can use this antibody to investigate the role of K29-linked ubiquitin chains in cellular processes and disease pathways.Overall, the K29 Linkage-Specific Polyubiquitin Rabbit Polyclonal Antibody (CAB18198) provides a valuable tool for elucidating the complex mechanisms of protein ubiquitination and its implications in health and disease.
Product Name:
K29-linkage Specific Polyubiquitin Rabbit Polyclonal Antibody
SKU:
CAB18198
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic peptide corresponding to a sequence containing polyubiquitinated protein (K29 linkage).
Polyubiquitin chains regulate diverse cellular processes through the ability of ubiquitin to form chains of eight different linkage types. when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked. K29-linked ubiquitination is involved in different kinds of cellular proteotoxic stress response as well as cell cycle regulation. In particular, K29-linked ubiquitination is enriched in the midbody and downregulation of the K29-linked ubiquitination signal arrests cells in G1/S phase.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using K29-linkage Specific Polyubiquitin Rabbit pAb (CAB18198) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
