K27-linkage Specific Polyubiquitin Rabbit Polyclonal Antibody (CAB18202)
The K27 Linkage-Specific Polyubiquitin Rabbit Polyclonal Antibody (CAB18202) is a valuable tool for researchers studying the ubiquitin signaling pathway. This antibody recognizes the K27-linked polyubiquitin chains, which play a key role in various cellular processes such as DNA repair, protein degradation, and signal transduction.Raised in rabbits, this antibody is highly specific and sensitive for detecting K27-linked polyubiquitin chains in human samples. Validated for use in Western blot applications, it allows for the precise detection and quantification of these specific ubiquitin chains in different cell types and tissues.
This makes it an essential tool for investigating the role of K27-linked polyubiquitin chains in biological processes related to cancer, neurodegenerative diseases, and immune system regulation.By targeting the K27 linkage-specific polyubiquitin chains, researchers can gain insights into the mechanisms underlying these cellular processes and potentially identify novel therapeutic targets for various diseases. The CAB18202 antibody opens up new avenues for research in the field of ubiquitin biology and holds great promise for advancing our understanding of cellular signaling pathways.
Product Name:
K27-linkage Specific Polyubiquitin Rabbit Polyclonal Antibody
SKU:
CAB18202
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence containing polyubiquitinated protein (K27 linkage).
Tested Applications:
WBELISADB
Recommended Dilution:
WB,1:500 - 1:2000 DB,1:500 - 1:1000
Positive Sample:
NIH/3T3,RAW264.7,C6,Mouse brain,Mouse kidney
Ubiquitination, one type of the most common post-translational modification, mediates the regulation of protein homeostasis in vivo. Since ubiquitin itself contains multiple lysine residues and one N-terminal free amino group, eight types of ubiquitin chains can be formed. The K27 ubiquitin chain is formed through the ubiquitination of the ubiquitin Lys27 (K27), which adopts a compact conformation. In recent years, biological function of the K27 ubiquitin chain in innate immunity, protein homeostasis and DNA damage has been discovered, but the molecular mechanisms of K27 ubiquitin chain assembly, recognition and hydrolysis are still poorly understood.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using K27-linkage Specific Polyubiquitin Rabbit pAb (CAB18202) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
