The Junctophilin-4 Polyclonal Antibody (CAB10333) is a valuable tool for researchers studying Junctophilin-4, a protein involved in maintaining calcium homeostasis and facilitating communication between the plasma membrane and the endoplasmic reticulum in muscle cells. This antibody, produced in rabbits, is highly specific for detecting Junctophilin-4 in human samples and has been validated for use in Western blot applications.Junctophilin-4 plays a crucial role in the muscle contraction process by mediating the formation of junctions between the plasma membrane and the sarcoplasmic reticulum.
Dysregulation of Junctophilin-4 has been implicated in muscle diseases such as muscular dystrophy and heart failure, making it a promising target for therapeutic interventions.By using the Junctophilin-4 Polyclonal Antibody, researchers can further explore the functions and molecular mechanisms of Junctophilin-4 in muscle physiology and pathology. This antibody offers a reliable tool for analyzing Junctophilin-4 expression in various cell types and is essential for advancing our understanding of muscle-related diseases.
Product Name:
JPH4 Rabbit Polyclonal Antibody
SKU:
CAB10333
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 405-605 of human JPH4 (NP_115828.2).
Cell membrane, Endoplasmic reticulum membrane, Peripheral membrane protein, Single-pass type IV membrane protein.
Calculated MW:
66kDa
Observed MW:
85kDa
This gene encodes a member of the junctophilin family of transmembrane proteins that are involved in the formation of the junctional membrane complexes between the plasma membrane and the endoplasmic/sarcoplasmic reticulum in excitable cells. The encoded protein contains a conserved N-terminal repeat region called the membrane occupation and recognition nexus sequence that is found in other members of the junctophilin family. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
84502
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using JPH4 antibody (CAB10333) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
