JNK1/2/3 Polyclonal Antibody (CAB18678)
- SKU:
- CAB18678
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- IHC
- IF
- IP
Description
| Product Name: | JNK1/2/3 Polyclonal Antibody |
| SKU: | CAB18678 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human JNK1/2/3 (NP_620637.1). |
| Sequence: | MSRS KRDN NFYS VEIG DSTF TVLK RYQN LKPI GSGA QGIV CAAY DAIL ERNV AIKK LSRP FQNQ THAK RAYR ELVL MKCV NHKN IIGL LNVF TPQK SLEE FQDV YIVM ELMD ANLC QVIQ MELD HERM SYLL YQML CGIK HLHS AGII HRDL KPSN IVVK SDCT LKIL DFGL ARTA GTSF MMTP YVVT RYYR APEV ILGM GYKE NVDL WSVG CIMG EMVC HKIL FPGR DYID QWNK VIEQ LGTP CPEF MKKL QPTV RTYV ENRP KYAG YSFE KLFP DVLF PADS EHNK LKAS QARD LLSK MLVI DASK RISV DEAL QHPY INVW YDPS EAEA PPPK IPDK QLDE REHT IEEW KELI YKEV MDLE ERTK NG |
| Tested Applications: | WB IHC-P IF/ICC IP ELISA |
| Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells |
| Positive Sample: | SH-SY5Y,Mouse brain,Rat brain |
| Cellular Localization: | axon, basal dendrite, cytoplasm, cytosol, mitochondrion, nucleoplasm, nucleus, synapse |
| Calculated MW: | 35kDa/44kDa/48kDa/27kDa/52kDa |
| Observed MW: | 46kDa/54kDa |
JNK belongs to MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1,JNK2,and JNK3
| Purification Method: | Affinity purification |
| Gene ID: | 5599/5601/5602 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using JNK1/2/3 Rabbit pAb (CAB18678) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s. |
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