The JAK2 Rabbit Monoclonal Antibody (CAB19629) is a powerful tool for researchers studying the JAK2 protein, a key player in cell signaling pathways and implicated in various diseases, including cancer and autoimmune disorders. This antibody, developed using rabbit monoclonal technology, shows high specificity and sensitivity for detecting JAK2 in human samples, making it an ideal choice for Western blot applications.JAK2, a member of the Janus kinase family, plays a crucial role in regulating cell growth, differentiation, and survival, making it a promising target for therapeutic intervention.
Dysregulation of JAK2 signaling has been linked to various malignancies and hematologic disorders, highlighting its importance in disease pathology. By using the JAK2 Rabbit Monoclonal Antibody, researchers can explore the function and regulation of JAK2 in different cell types and disease contexts, advancing our understanding of its role in health and disease.
Product Name:
JAK2 Rabbit Monoclonal Antibody
SKU:
CAB19629
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1033-1132 of human JAK2 (O60674).
This gene encodes a non-receptor tyrosine kinase that plays a central role in cytokine and growth factor signalling. The primary isoform of this protein has an N-terminal FERM domain that is required for erythropoietin receptor association, an SH2 domain that binds STAT transcription factors, a pseudokinase domain and a C-terminal tyrosine kinase domain. Cytokine binding induces autophosphorylation and activation of this kinase. This kinase then recruits and phosphorylates signal transducer and activator of transcription (STAT) proteins. Growth factors like TGF-beta 1 also induce phosphorylation and activation of this kinase and translocation of downstream STAT proteins to the nucleus where they influence gene transcription. Mutations in this gene are associated with numerous inflammatory diseases and malignancies. This gene is a downstream target of the pleiotropic cytokine IL6 that is produced by B cells, T cells, dendritic cells and macrophages to produce an immune response or inflammation. Disregulation of the IL6/JAK2/STAT3 signalling pathways produces increased cellular proliferation and myeloproliferative neoplasms of hematopoietic stem cells. A nonsynonymous mutation in the pseudokinase domain of this gene disrupts the domains inhibitory effect and results in constitutive tyrosine phosphorylation activity and hypersensitivity to cytokine signalling. This gene and the IL6/JAK2/STAT3 signalling pathway is a therapeutic target for the treatment of excessive inflammatory responses to viral infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
3717
Clone Number:
ARC0108
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using JAK2 Rabbit mAb (CAB19629) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 30s.
