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Anti-JAK2 Antibody (CAB11497)

SKU:
CAB11497
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
  • Immunology Antibodies 1 Anti-JAK2 Antibody CAB11497
  • Immunology Antibodies 1 Anti-JAK2 Antibody CAB11497
  • Immunology Antibodies 1 Anti-JAK2 Antibody CAB11497
  • Immunology Antibodies 1 Anti-JAK2 Antibody CAB11497
  • Immunology Antibodies 1 Anti-JAK2 Antibody CAB11497
€139 - €419
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Description

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Anti-JAK2 Antibody (CAB11497)

The JAK2 Polyclonal Antibody (CAB11497) is specifically designed for research involving JAK2, a key protein kinase involved in signaling pathways that regulate cell growth, survival, and differentiation. This antibody, generated in rabbits, is highly reactive with human samples and is validated for use in Western blot applications. By binding to JAK2, this antibody allows for the detection and analysis of JAK2 protein levels in various cell types, making it ideal for studies in cell signaling, oncology, and hematopoiesis.JAK2 is a critical player in the JAK/STAT signaling pathway, which is involved in various cellular processes including immune response, hematopoiesis, and inflammation.

Dysregulation of JAK2 activity has been linked to a variety of diseases, including myeloproliferative disorders and certain types of leukemia. The ability to study and understand the role of JAK2 in these diseases is essential for the development of targeted therapies and treatments.In summary, the JAK2 Polyclonal Antibody (CAB11497) provides researchers with a valuable tool for studying the function and regulation of JAK2 in biological processes and disease conditions, ultimately contributing to advancements in medicine and therapeutic interventions.

Antibody Name:Anti-JAK2 Antibody
Antibody SKU:CAB11497
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human JAK2
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:C6, Mouse brain, Mouse lung
Immunogen:A synthetic peptide of human JAK2
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:3717
Uniprot:O60674
Cellular Location:Cytoplasm, Endomembrane system, Nucleus, Peripheral membrane protein
Calculated MW:130kDa
Observed MW:111KDa
Synonyms:JAK2, JTK10, THCYT3
Background:This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.
UniProt Protein Function:JAK2: a non-receptor tyrosine-kinase involved in a specific subset of cytokine receptor signaling pathways, including IL-3, -5 and GM-CSF. Interacts with IL23R, SKB1 and STAM2. It has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. Fusion of Jak2 to TEL1 (ETV6) by t(9;12)(p24;p13) causes myeloproliferative disease in humans and mouse models. The Jak inhibitor AG490 inhibits constitutive Jak2 phosphorylation and causes apoptosis in cells from breast cancer and relapsing acute lymphoblastic leukemia. A single activating mutation is associated with several hematological malignancies. Inhibitor: AG490.
UniProt Protein Details:

Protein type:Protein kinase, TK; EC 2.7.10.2; Kinase, protein; Oncoprotein; Protein kinase, tyrosine (non-receptor); TK group; JakA family

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: caveola; cytoplasm; cytoskeleton; cytosol; extrinsic to internal side of plasma membrane; lipid raft; nuclear matrix; nucleoplasm; nucleus

Molecular Function:acetylcholine receptor binding; ATP binding; growth hormone receptor binding; heme binding; histone binding; insulin receptor substrate binding; interleukin-12 receptor binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; peptide hormone receptor binding; phosphoinositide 3-kinase binding; protein binding; protein C-terminus binding; protein kinase activity; protein kinase binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor binding; SH2 domain binding; type 1 angiotensin receptor binding

Biological Process: actin filament polymerization; activation of MAPKK activity; adaptive immune response; apoptosis; axon regeneration; blood coagulation; caspase activation; cell differentiation; cell migration; cell motility; cytokine and chemokine mediated signaling pathway; elevation of cytosolic calcium ion concentration; enzyme linked receptor protein signaling pathway; erythrocyte differentiation; G-protein coupled receptor protein signaling pathway; induction of apoptosis by oxidative stress; innate immune response; JAK-STAT cascade; MAPKKK cascade; mesoderm development; mineralocorticoid receptor signaling pathway; negative regulation of cell proliferation; negative regulation of cell-cell adhesion; negative regulation of DNA binding; negative regulation of heart contraction; negative regulation of neuron apoptosis; peptidyl-tyrosine phosphorylation; platelet-derived growth factor receptor signaling pathway; positive regulation of cell activation; positive regulation of cell differentiation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of GTPase activity; positive regulation of inflammatory response; positive regulation of insulin secretion; positive regulation of interleukin-1 beta production; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of phosphoprotein phosphatase activity; positive regulation of protein import into nucleus, translocation; positive regulation of transcription factor activity; positive regulation of tumor necrosis factor production; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of tyrosine phosphorylation of Stat5 protein; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of cell proliferation; regulation of inflammatory response; response to antibiotic; response to hydroperoxide; response to lipopolysaccharide; signal transduction; STAT protein nuclear translocation; tumor necrosis factor-mediated signaling pathway; tyrosine phosphorylation of JAK2 protein; tyrosine phosphorylation of STAT protein; tyrosine phosphorylation of Stat1 protein; tyrosine phosphorylation of Stat3 protein; tyrosine phosphorylation of Stat5 protein

Disease: Budd-chiari Syndrome; Erythrocytosis, Familial, 1; Myelofibrosis; Polycythemia Vera; Thrombocythemia 3

NCBI Summary:This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008]
UniProt Code:O60674
NCBI GenInfo Identifier:12643404
NCBI Gene ID:3717
NCBI Accession:O60674.2
UniProt Secondary Accession:O60674,O14636, O75297,
UniProt Related Accession:O60674
Molecular Weight:130,674 Da
NCBI Full Name:Tyrosine-protein kinase JAK2
NCBI Synonym Full Names:Janus kinase 2
NCBI Official Symbol:JAK2
NCBI Official Synonym Symbols:JTK10; THCYT3
NCBI Protein Information:tyrosine-protein kinase JAK2
UniProt Protein Name:Tyrosine-protein kinase JAK2
UniProt Synonym Protein Names:Janus kinase 2; JAK-2
Protein Family:Tyrosine-protein kinase
UniProt Gene Name:JAK2
UniProt Entry Name:JAK2_HUMAN
Anti-JAK2 Antibody (CAB11497)Western blot analysis of extracts of various cell lines, using JAK2 Rabbit pAb (CAB11497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
Anti-JAK2 Antibody (CAB11497)Immunohistochemistry of paraffin-embedded rat spleen using JAK2 Rabbit pAb (CAB11497) at dilution of 1:100 (40x lens).
Anti-JAK2 Antibody (CAB11497)Immunohistochemistry of paraffin-embedded human breast cancer using JAK2 Rabbit pAb (CAB11497) at dilution of 1:100 (40x lens).
Anti-JAK2 Antibody (CAB11497)Immunohistochemistry of paraffin-embedded human esophageal using JAK2 Rabbit pAb (CAB11497) at dilution of 1:100 (40x lens).
Anti-JAK2 Antibody (CAB11497)Immunohistochemistry of paraffin-embedded mouse stomach using JAK2 Rabbit pAb (CAB11497) at dilution of 1:100 (40x lens).

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