The ITSN1 Polyclonal Antibody (CAB18378) is a valuable tool for researchers studying the intracellular protein intersectin-1 (ITSN1) and its role in signaling pathways related to endocytosis and cell migration. This antibody, derived from rabbit sources, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the ITSN1 protein, researchers can accurately detect and analyze its expression in various cell types, providing valuable insights into its functions in cellular processes.ITSN1 is a multifunctional scaffold protein involved in the regulation of membrane dynamics and intracellular signaling cascades. Its interactions with numerous binding partners implicate ITSN1 in processes such as receptor trafficking, cytoskeletal organization, and cell adhesion.
Understanding the molecular mechanisms by which ITSN1 influences these pathways is essential for advancing knowledge in areas such as cell biology, neurobiology, and cancer research.The ITSN1 Polyclonal Antibody is a reliable tool for investigating the roles of ITSN1 in cellular physiology and pathology. With its high specificity and sensitivity, this antibody enables precise detection and analysis of ITSN1 protein levels, allowing researchers to uncover novel insights into its functions and potential implications in disease pathways. Whether studying endocytosis, cell migration, or signal transduction, this antibody is a valuable asset for exploring the multifaceted roles of ITSN1 in diverse biological contexts.
Product Name:
ITSN1 Rabbit Polyclonal Antibody
SKU:
CAB18378
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 750-850 of human ITSN1 (NP_001317941.1).
The protein encoded by this gene is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far.
Purification Method:
Affinity purification
Gene ID:
6453
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ITSN1 pAb (CAB18378) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
