The ITPA Polyclonal Antibody (CAB1221) is a highly specific antibody designed for research involving the inosine triphosphate pyrophosphatase (ITPA) protein. This antibody, raised in rabbits, is suitable for use in various applications, including Western blot and immunohistochemistry, and has been validated for its reactivity with human samples.ITPA is an enzyme that plays a crucial role in maintaining cellular homeostasis by regulating the levels of inosine triphosphate (ITP) in the cell. Mutations in the ITPA gene have been associated with various genetic disorders, making it a key target for research in the field of molecular biology and genetics.
By utilizing the ITPA Polyclonal Antibody, researchers can accurately detect and analyze the expression of ITPA in different cell types, providing valuable insights into its function and potential implications in disease pathogenesis. This antibody is an essential tool for studies aiming to elucidate the role of ITPA in cellular metabolism and genetic diseases, laying the foundation for the development of targeted therapies in the future.
Product Name:
ITPA Rabbit Polyclonal Antibody
SKU:
CAB1221
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human ITPA (NP_258412.1).
U-87MG,HT-29,Mouse small intestine,Mouse heart,Rat liver
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
21kDa
Observed MW:
21kDa
This gene encodes an inosine triphosphate pyrophosphohydrolase. The encoded protein hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. This protein, which is a member of the HAM1 NTPase protein family, is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency which causes an accumulation of ITP in red blood cells. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3704
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ITPA Rabbit pAb (CAB1221) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
