The ITK Monoclonal Antibody is a valuable tool for research involving ITK, a signaling molecule crucial for T cell activation and differentiation. This antibody, produced from mouse hybridoma cells, exhibits high specificity and sensitivity in detecting ITK in human samples, making it ideal for Western blot, immunohistochemistry, and flow cytometry applications.ITK, also known as interleukin-2-inducible T-cell kinase, is essential for T cell receptor signaling and immune response regulation. Its role in modulating T cell function makes it a key player in diseases such as cancer, autoimmune disorders, and infectious diseases.
By investigating the function of ITK, researchers can gain insights into how T cell activation is controlled and potentially identify new targets for therapeutic intervention.Overall, the ITK Monoclonal Antibody offers researchers a reliable tool for studying the role of ITK in immune response modulation and disease pathogenesis. Its specificity and sensitivity make it a valuable asset for immunology and cancer research, providing essential insights into the complex mechanisms of immune regulation.
Product Name:
ITK/EMT Monoclonal Antibody
SKU:
CAB22617
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant protein of human ITK/EMT.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:2000 - 1:6000
Synonyms:
EMT; LYK; LPFS1; PSCTK2; ITK/EMT
Positive Sample:
Jurkat,Molt-4
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
72kDa
Observed MW:
75kDa
This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.
Purification Method:
Affinity purification
Gene ID:
3702
Clone Number:
ARC58362
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using ITK/EMT antibody (CAB22617) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
