The ITGB3 Polyclonal Antibody (CAB0076) is specifically designed for research involving the integrin beta-3 protein, a cell surface receptor that plays a crucial role in cell adhesion and signaling. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in various applications, including Western blotting.Integrin beta-3 is involved in a wide range of physiological processes, including platelet aggregation, angiogenesis, and immune response regulation. Dysregulation of integrin beta-3 has been implicated in diseases such as thrombosis, atherosclerosis, and cancer, making it an important target for further research.
By using the ITGB3 Polyclonal Antibody, researchers can effectively detect and analyze integrin beta-3 expression in different cell types, providing valuable insights into its role in health and disease. This antibody is a valuable tool for studies in cell biology, immunology, and cancer research, enabling a better understanding of integrin beta-3 function and its potential as a therapeutic target.
Cell junction, Cell membrane, Cell projection, Single-pass type I membrane protein, focal adhesion, lamellipodium membrane.
Calculated MW:
87kDa
Observed MW:
97kDa
The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
Purification Method:
Affinity purification
Gene ID:
3690
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Integrin beta 3 (ITGB3/CD61) antibody (CAB0076) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
