Itgb1 Antibody (PACO27689)
- SKU:
- PACO27689
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
Itgb1 Antibody (PACO27689)
The ITGB1 Polyclonal Antibody (PAC027689) is a valuable tool for research involving integrin beta-1 (ITGB1), a cell adhesion molecule crucial for cell migration, proliferation, and differentiation. This antibody, raised in rabbits, exhibits high specificity and sensitivity when used with human samples in Western blot applications. By binding to the ITGB1 protein, this antibody enables accurate detection and analysis of ITGB1 expression in various cell types, making it an ideal choice for studies in cell biology, cancer research, and tissue engineering.
ITGB1 plays a pivotal role in cell-extracellular matrix interactions, signal transduction, and cell adhesion processes essential for tissue development and repair. Dysregulation of ITGB1 expression and function has been implicated in various diseases, including cancer, cardiovascular disorders, and inflammatory conditions. Investigating the role of ITGB1 in these contexts is crucial for identifying potential therapeutic targets and developing novel treatment strategies.
| Antibody Name: | Itgb1 Antibody (PACO27689) |
| Antibody SKU: | PACO27689 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IF:1:50-1:200 |
| Species Reactivity: | Mouse, Human |
| Immunogen: | Recombinant Mouse Integrin beta-1 protein (21-728AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescent analysis of Hela cells using PACO27689 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Western blot All lanes: Itgb1 antibody at 16µg/ml + U87 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 89 kDa Observed band size: 89 kDa . |
| Background: | Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1 and recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (By similarity). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (By similarity). |
| Synonyms: | Integrin beta-1 (Fibronectin receptor subunit beta) (VLA-4 subunit beta) (CD antigen CD29), Itgb1 |
| UniProt Protein Function: | ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin.. Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle). |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Cell adhesion; Receptor, misc.; Motility/polarity/chemotaxis; Cell surface Cellular Component: focal adhesion; cell surface; dendritic spine; integral to membrane; acrosome; intercellular junction; lipid raft; cell projection; adherens junction; hemidesmosome; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; basement membrane; synapse; cytoplasmic vesicle; neuromuscular junction; cell junction; integrin complex; receptor complex; sarcolemma; endosome; filopodium; external side of plasma membrane Molecular Function:protein domain specific binding; protease binding; metal ion binding; laminin binding; receptor activity; actin binding; alpha-actinin binding; peptide binding; protein kinase binding; collagen binding; integrin binding; protein binding; protein heterodimerization activity; fibronectin binding; protein complex binding; cell adhesion molecule binding; glycoprotein binding; kinase binding; receptor binding Biological Process: positive regulation of apoptosis; axon extension; regulation of cell cycle; multicellular organismal development; cell-matrix adhesion; cell fate specification; positive regulation of endocytosis; cardiac muscle cell differentiation; negative regulation of cell proliferation; leukocyte adhesion; positive regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; germ cell migration; positive regulation of cell proliferation; tissue homeostasis; visual learning; cell adhesion; negative regulation of cell projection organization and biogenesis; neurite development; integrin-mediated signaling pathway; cardiac muscle development; cell migration; in utero embryonic development; dendrite morphogenesis; sarcomere organization; protein transport within lipid bilayer; cell-substrate adhesion; formation of radial glial scaffolds; cellular calcium ion homeostasis; heterotypic cell-cell adhesion; negative regulation of cell differentiation; negative regulation of neuron differentiation; positive regulation of peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; negative regulation of Rho protein signal transduction; calcium-independent cell-matrix adhesion; stress fiber formation; positive regulation of neuron differentiation; leukocyte tethering or rolling; regulation of G-protein coupled receptor protein signaling pathway; G1/S transition of mitotic cell cycle; positive regulation of cell migration |
| UniProt Code: | P09055 |
| NCBI GenInfo Identifier: | 124964 |
| NCBI Gene ID: | 16412 |
| NCBI Accession: | P09055.1 |
| UniProt Secondary Accession: | P09055,Q3TIW5, Q3TWH6, Q60993, Q61126, Q8BTU0, Q8BVU1 F6R105, |
| UniProt Related Accession: | P09055 |
| Molecular Weight: | 88,700 Da |
| NCBI Full Name: | Integrin beta-1 |
| NCBI Synonym Full Names: | integrin beta 1 (fibronectin receptor beta) |
| NCBI Official Symbol: | Itgb1Â Â |
| NCBI Official Synonym Symbols: | CD29; Fnrb; gpIIa; Gm9863; AA409975; AA960159; 4633401G24Rik; ENSMUSG00000051907Â Â |
| NCBI Protein Information: | integrin beta-1; VLA-4 subunit beta; fibronectin receptor subunit beta |
| UniProt Protein Name: | Integrin beta-1 |
| UniProt Synonym Protein Names: | Fibronectin receptor subunit beta; VLA-4 subunit beta; CD_antigen: CD29 |
| Protein Family: | Fibronectin |
| UniProt Gene Name: | Itgb1Â Â |
| UniProt Entry Name: | ITB1_MOUSE |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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