The ITCH Polyclonal Antibody (CAB15612) is a valuable tool for researchers studying the ITCH protein, a critical regulator of cellular processes such as protein degradation and immune response modulation. This antibody, generated in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By binding to the ITCH protein, this antibody allows for precise detection and analysis in various cell types, making it ideal for use in immunology and cancer research studies.
ITCH, also known as E3 ubiquitin-protein ligase, plays a crucial role in maintaining cellular homeostasis by targeting specific proteins for degradation and regulating immune responses. Its involvement in these pathways makes it a promising target for research into diseases such as cancer, autoimmune disorders, and inflammatory conditions. Understanding the function of ITCH is essential for developing therapies that can modulate immune responses and potentially treat these diseases effectively.
Product Name:
[KO Validated] ITCH Rabbit Polyclonal Antibody
SKU:
CAB15612
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-155 of ITCH (NP_001244066.1).
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
83737
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] ITCH Rabbit pAb (CAB15612) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
