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Isocitrate Assay Kit (Fluorometric) (BA0103)

SKU:
BA0103
Product Type:
Assay
Detection Method:
Fluorometric
Instrument:
Microplate Reader
Sample Type:
Food, Beverage, Biological Samples
Research Area:
TCA Cycle
Diabetes & Obesity
Food Safety & Analysis
€879
Frequently bought together:

Description

ELISA Kit Technical ManualMSDS

Isocitrate Assay Kit

ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing alpha-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.

Assay Genie's isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at Ex/Em = 530/585 nm, is proportional to the isocitrate concentration in the sample.

Applications

For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.

Key Features

  • Fast and sensitive. Linear detection range (20 uL sample): 0.6 to 500 uM for 10 min reaction.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Isocitrate Assay Kit Information:

Kit Includes: Assay Buffer: 10 mL Enzyme A: 120 uL NADP: 500 uL Enzyme B: 120 uL Standard: 1 mL Probe: 750 uL
Kit Requires: Pipetting devices and accessories (e.g. multi-channel pipettor), black flat-bottom 96-well plates, centrifuge tubes and fluorescence plate reader capable of reading at EX/EM= 530/585 nm.
Method of Detection: FL530/585 nm
Detection Limit: 0.6 uM
Samples: Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
Species: All
Protocol Length: 10 min
Size: 100 tests
Storage: Store all components at -20°C upon receiving
Shelf Life: 6 months

 

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