Isocitrate Assay Kit (Colorimetric) (BA0089)

SKU:: BA0089
UPC:
Product Type:: Assay
Detection Method:: Colorimetric
Instrument:: Microplate Reader
Sample Type:: Food, Beverage, Cells, Tissue
Research Area:: Ferroptosis; TCA Cycle; Lipid Metabolism; Diabetes & Obesity; Food Safety & Analysis; Plant & Environmental Stress

$1,055

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Description

Description

Isocitrate Assay Kit - Information

Assay Genie's isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the isocitrate concentration.

Applications

For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.

Isocitrate Assay Kit - Key Features

Fast and sensitive. Linear detection range (20 sample): 20 to 5000 for 10 min reaction.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Isocitrate Assay Kit - Data Sheet
Method of Detection	OD565nm
Detection Limit	20
Samples	Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
Species	All
Protocol Length	10 min
Size	100 tests
Shelf Life	6 months

More Details

ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing alpha-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.