The ISL1 Polyclonal Antibody (CAB6383) is a valuable tool for researchers studying ISL1, a transcription factor essential for the development of the heart, pancreas, and nervous system. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blotting applications. By targeting the ISL1 protein, researchers can gain valuable insights into its role in various cellular processes, making it an ideal choice for studies in developmental biology and regenerative medicine.
ISL1, also known as insulin gene enhancer protein ISL-1, plays a crucial role in the early development of multiple organs and tissues. Its involvement in regulating gene expression and cell differentiation makes it a key target for research into congenital heart defects, diabetes, and neurological disorders. Understanding the function of ISL1 is essential for uncovering potential therapeutic strategies for treating these conditions and advancing our knowledge of developmental biology.
Product Name:
Islet1 Rabbit Polyclonal Antibody
SKU:
CAB6383
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human Islet1 (NP_002193.2).
This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.
Purification Method:
Affinity purification
Gene ID:
3670
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Islet1 antibody (CAB6383) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
