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ISG15 Rabbit Monoclonal Antibody (CAB2416)

SKU:
CAB2416
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
€139 - €419
Frequently bought together:

Description

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Product Name:ISG15 Rabbit Monoclonal Antibody
SKU:CAB2416
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human ISG15 (NP_005092.1).
Sequence:MGWD LTVK MLAG NEFQ VSLS SSMS VSEL KAQI TQKI GVHA FQQR LAVH PSGV ALQD RVPL ASQG LGPG STVL LVVD KCDE PLSI LVRN NKGR SSTY EVRL TQTV AHLK QQVS GLEG VQDD LFWL TFEG KPLE DQLP LGEY GLKP LSTV FMNL RLRG GGTE PGGR S
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:G1P2; IP17; UCRP; IFI15; IMD38; hUCRP; [KD Validated] ISG15
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Secreted.
Calculated MW:18kDa
Observed MW:15kDa

The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.

Purification Method:Affinity purification
Gene ID:9636
Clone Number:ARC53794
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type(WT) and ISG15 knockdown (KD) 293T cells, using [KD Validated] ISG15 Rabbit mAb (CAB2416) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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