The ISCU Polyclonal Antibody (CAB7082) is a valuable tool for researchers studying the ISCU protein, which is involved in iron-sulfur cluster assembly. This antibody, produced in rabbits, shows high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the ISCU protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in molecular biology and biochemistry.The ISCU protein plays a crucial role in cellular processes related to iron-sulfur cluster biogenesis, which are essential for various biological functions such as mitochondrial respiration and DNA repair.
Dysregulation of ISCU has been linked to disorders like Friedreich's ataxia and other mitochondrial diseases, making it a promising target for therapeutic interventions. By investigating the function of ISCU, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially develop new treatments to address them.
Product Name:
ISCU Rabbit Polyclonal Antibody
SKU:
CAB7082
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human ISCU (NP_998760.1).
This gene encodes a component of the iron-sulfur (Fe-S) cluster scaffold. Fe-S clusters are cofactors that play a role in the function of a diverse set of enzymes, including those that regulate metabolism, iron homeostasis, and oxidative stress response. Alternative splicing results in transcript variants encoding different protein isoforms that localize either to the cytosol or to the mitochondrion. Mutations in this gene have been found in patients with hereditary myopathy with lactic acidosis. A disease-associated mutation in an intron may activate a cryptic splice site, resulting in the production of a splice variant encoding a putatively non-functional protein. A pseudogene of this gene is present on chromosome 1.
Purification Method:
Affinity purification
Gene ID:
23479
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ISCU Antibody (CAB7082) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 10s.
