The IRX4 Polyclonal Antibody (PAC054314) is a valuable tool for researchers studying the IRX4 protein, a transcription factor essential for heart development and function. This antibody, generated in rabbits, exhibits high specificity and sensitivity in detecting IRX4 in human samples, making it suitable for use in Western blot and immunohistochemistry applications.IRX4 is a crucial regulator of cardiac gene expression and plays a pivotal role in the development and maintenance of the heart. Dysregulation of IRX4 has been linked to various cardiac disorders, including arrhythmias and heart failure, making it a promising target for therapeutic interventions.
By accurately detecting and quantifying IRX4 levels, researchers can gain insights into its role in cardiac biology and potentially discover new treatment strategies for heart-related diseases.Overall, the IRX4 Polyclonal Antibody offers researchers a reliable tool to investigate the functions and mechanisms of IRX4 in cardiac development and disease, paving the way for advancements in the field of cardiovascular research.
Western Blot. Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, Colo320 whole cell lysate. All lanes: IRX4 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55, 58 kDa. Observed band size: 55 kDa.
Immunofluorescence staining of MCF-7 cells with PACO54314 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Likely to be an important mediator of ventricular differentiation during cardiac development.
Synonyms:
Iroquois-class homeodomain protein IRX-4 (Homeodomain protein IRXA3) (Iroquois homeobox protein 4), IRX4, IRXA3
UniProt Protein Function:
Likely to be an important mediator of ventricular differentiation during cardiac development.
