The IRF5 Rabbit Monoclonal Antibody is a highly specific antibody designed for research involving the IRF5 protein, a key regulator of immune responses. This antibody, raised in rabbits, is validated for use in various applications such as Western blotting, immunohistochemistry, and immunofluorescence. It detects the IRF5 protein in human samples, enabling researchers to study its role in immune system function and inflammatory pathways.IRF5 is a transcription factor that plays a crucial role in the activation of inflammatory responses and immune cell differentiation. Dysregulation of IRF5 has been linked to autoimmune disorders, infectious diseases, and inflammatory conditions.
By targeting IRF5 with this antibody, researchers can better understand the molecular mechanisms underlying these diseases and potentially develop targeted therapies.With its high specificity and sensitivity, the IRF5 Rabbit Monoclonal Antibody is a valuable tool for immunology and inflammation research, providing insights into the complex interplay between immune signaling pathways and disease progression. It is an essential component for any laboratory studying the intricate nature of immune regulation and inflammatory disorders.
Product Name:
IRF5 Rabbit Monoclonal Antibody
SKU:
CAB11106
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 6-151 of human IRF5 (Q13568).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:
SLEB10; IRF5
Positive Sample:
293T,Jurkat,Mouse lung,Mouse spleen,Mouse thymus
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
56kDa
Observed MW:
60kDa
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment.
Purification Method:
Affinity purification
Gene ID:
3663
Clone Number:
ARC0525
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using IRF5 Rabbit mAb (CAB11106) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 5min.
