The IRF5 Antibody (CAB1149) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically targets the IRF5 protein, a key player in the regulation of immune responses and the inflammatory process. Validated for use in Western blot applications, the IRF5 Polyclonal Antibody is highly reactive with human samples, making it an essential tool for studies in immunology and autoimmune diseases.IRF5, also known as interferon regulatory factor 5, is a transcription factor that plays a crucial role in the activation of immune responses and the production of pro-inflammatory cytokines. Dysregulation of IRF5 has been implicated in various autoimmune disorders, making it a promising target for drug development and precision medicine approaches.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
IRF5 Antibody
SKU:
CAB1149
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SLEB10, IRF5
Positive Sample:
THP-1, U-937, Rat thymus
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
56kDa
Observed MW:
56kDa/60kDa
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment.
Purification Method
Affinity purification
Gene ID
3663
RRID
AB_2758581
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using IRF5 Rabbit pAb (CAB1149) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Western blot analysis of lysates from 293T cells, using IRF5 Rabbit pAb (CAB1149) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
