The IREB2 Polyclonal Antibody (CAB6382) is a valuable tool for researchers studying the IREB2 protein, which plays a key role in iron metabolism and regulation. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the IREB2 protein, this antibody allows for the detection and analysis of IREB2 in various cell types, making it ideal for investigations in areas such as molecular biology and hematology.IREB2, also known as iron-responsive element-binding protein 2, is essential for the control of cellular iron homeostasis and the regulation of iron absorption and distribution in the body.
Dysregulation of IREB2 has been implicated in diseases such as iron overload disorders and anemia. Therefore, studying the function and expression of IREB2 is crucial for understanding the molecular mechanisms underlying these conditions and developing targeted therapies.Overall, the IREB2 Polyclonal Antibody (CAB6382) offers researchers a reliable tool for exploring the role of IREB2 in iron metabolism and related disorders, paving the way for advancements in the field of iron biology and potential therapeutic strategies.
Product Name:
IREB2 Rabbit Polyclonal Antibody
SKU:
CAB6382
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 760-963 of human IRP2 (NP_004127.1).
The protein encoded by this gene is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner. Variants in this gene have been associated with lung cancer and chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
3658
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using IRP2 antibody (CAB6382) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
