The IRE1 Monoclonal Antibody is a vital tool for researchers studying the IRE1 protein, a key regulator of the unfolded protein response (UPR) in cells. This antibody, developed through monoclonal technology, offers high specificity and sensitivity for detecting IRE1 in various cell types and tissues. Validated for use in applications such as Western blot and immunofluorescence, it enables precise identification and analysis of IRE1's role in cellular stress responses.IRE1, a transmembrane protein located in the endoplasmic reticulum, is essential for maintaining protein homeostasis and cell survival under conditions of stress.
Dysregulation of the UPR, in which IRE1 plays a crucial role, has been implicated in various diseases including cancer, neurodegenerative disorders, and metabolic conditions. Therefore, investigating the function of IRE1 with this monoclonal antibody is crucial for understanding its potential as a therapeutic target in these pathologies.
Product Name:
IRE1 Monoclonal Antibody
SKU:
CAB21021
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 286-435 of human IRE1 (NP_001424.3).
Endoplasmic reticulum membrane, Single-pass type I membrane protein.
Calculated MW:
110kDa
Observed MW:
130kDa
This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.
Purification Method:
Affinity purification
Gene ID:
2081
Clone Number:
ARC57190
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using IRE1 antibody (CAB21021) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
