The IQGAP1 Polyclonal Antibody (CAB5679) is a valuable tool for researchers studying IQGAP1, a scaffold protein involved in cell signaling and cytoskeletal dynamics. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting IQGAP1 in human samples, making it ideal for use in Western blot applications. By binding to IQGAP1, researchers can analyze its expression and function in various cell types, providing insights into its role in cell signaling pathways and its potential implications in cancer and other diseases.
IQGAP1 is a multifunctional protein that regulates numerous cellular processes, including cell adhesion, migration, and proliferation. Its dysregulation has been associated with tumorigenesis and metastasis, highlighting its importance as a therapeutic target in cancer research. By investigating the function of IQGAP1 using this antibody, researchers can gain a deeper understanding of its impact on cellular behavior and its potential as a target for developing novel cancer treatments.
Product Name:
IQGAP1 Rabbit Polyclonal Antibody
SKU:
CAB5679
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 500-750 of human IQGAP1 (NP_003861.1).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
SAR1; p195; HUMORFA01; IQGAP1
Positive Sample:
A-549,293T,HepG2,HT-29,HeLa,293F
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane.
Calculated MW:
189kDa
Observed MW:
189kDa/189kDa
This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.
Purification Method:
Affinity purification
Gene ID:
8826
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using IQGAP1 Rabbit pAb (CAB5679) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
